Biocompatibility study of surface-grafted proteins on biomedical device
Question
How can we compare the biocompatibility of 2 différents grafting for intended application? Are the molecular conformation and orientation of the protein appropriate?
Are the amino-acids which induce cell growth predominantly present at the extreme surface?
Expertise
Materia Nova has extensive experience in extreme surface analysis using ToF-SIMS (approximate analysis depth of 1nm).
ToF-SIMS uses molecular information and high mass resolution to determine which amino acid coming from protein are mainly detected on the surface.
As the ToF-SIMS spectra of proteins are very complex, their interpretion is made using PCA (Principal Component Analysis) following litterature [Bertrand, P. Applied Surface Science 2006, 252, 6986]
Solution
ToF-SIMS analysis coupled with PCA treatement enables us to confirm the good orientation of the grafted-proteins of device n°1 and to validate its biocompatibility.